Preprocessing of 2-Dimensional Gel Electrophoresis Images Applied to Proteomic Analysis: A Review
نویسندگان
چکیده
منابع مشابه
Proteomic Profile in Glomeruli of Type-2 Diabetic KKAy Mice using 2-Dimensional Differential Gel Electrophoresis
BACKGROUND Diabetic nephropathy (DN) is a leading cause of end-stage renal disease. To search for glomerular proteins associated with early-stage DN, glomeruli of spontaneous type 2 diabetic KKAy mice were analyzed by 2-dimensional differential gel electrophoresis (2D-DIGE). MATERIAL AND METHODS Glomeruli of 20-week spontaneous type 2 diabetic KKAy mice and age-matched C57BL/6 mice were isola...
متن کاملSemiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis method.
More than 200 proteins associate with human spliceosomes, but little is known about their relative abundances in a given spliceosomal complex. Here we describe a novel two-dimensional (2D) electrophoresis method that allows separation of high-molecular-mass proteins without in-gel precipitation and thus without loss of protein. Using this system coupled with mass spectrometry, we identified 171...
متن کاملProteomic analysis of a highly metastatic gastric cancer cell line using two-dimensional differential gel electrophoresis.
Stomach cancer is still a major cause of death in Asian people despite a complete cure after the resection of early cancers, mainly because peritoneal dissemination is difficult to treat. In the present study, we used two-dimensional differential gel electrophoresis (2-D DIGE) to identify specific proteins differentially expressed between a highly metastatic stomach cancer cell line MKN-45-P an...
متن کاملTwo-Dimensional Gel Electrophoresis (2-DE)
Two-dimensional gel electrophoresis (2-DE) is able to separate hundreds to thousands of proteins or polypeptides by coupling IsoElectric Focusing (IEF) in first dimension and Sodium Dodecyl Sulphate PolyAcrylamide-Gel Electrophoresis (SDS-PAGE) in second dimension. This particular configuration is called classical 2-DE: IEF separates proteins in function of their isoelectric point (pI) and SDS-...
متن کاملOptimization of 2-dimensional gel electrophoresis for proteomic studies of solid tumor tissue samples.
The method of 2‑dimensional gel electrophoresis (2-DE) has been widely used for the proteomic profiling of solid biological samples, however, the analytical conditions have not been optimized. The present study optimized the major conditions of 2‑DE for determining the protein contents of solid tumor tissues, through enhancement of the separation efficiency and resolution. Three major analytica...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genomics, Proteomics & Bioinformatics
سال: 2018
ISSN: 1672-0229
DOI: 10.1016/j.gpb.2017.10.001